Genome-wide primer design
Upload Target(s) file
Upload target(s) fasta file :
Example target fasta file
Provide target sequence(s):
Provide target sequences in FASTA format.
First line with ">"
Upload Primer3 parameter file
Upload Primer3 parameter file :
Primer3 full parameter file
Compatible with Primer3web Version 4.1.0
Overwrite Primer3 file's parameter with below parameters
:
If user select this option, then Primer3 file's Parameter
will be over written with parameters provided below.
Parameters for primer selection
Pick left primer, or use left primer below:
Pick hybridization probe or use oligo below:
Pick right primer, or use right primer below:
Product Size Ranges
:
Primer pair to return
:
Targets
:
Included Region
:
Excluded Regions
:
Pair OK Region List
:
Product size
:
Min:
Opt:
Max:
Primer Tm
:
Min:
Opt:
Max:
Max Tm Difference:
Primer size
:
Min:
Opt:
Max:
Primer GC%
:
Min:
Opt:
Max:
Parameters for Hyb Oligo (Internal Oligo) selection
Hyb Proble Size :
Min:
Opt:
Max:
Hyb Probe Tm :
Min:
Opt:
Max:
Hyb Probe GC% :
Min:
Opt:
Max:
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