AVALON Primer specificity analysis
Provide primer pairs
Select primer pair file : 
Forward Primer Reverse Primer Delete primer pairs rows Add primer pairs rows?
Max. mismatches: 
Give maximum number of mismatches for blast search 
Min. product length: 
Give the minimum primer product length 
Max. product length: 
Give the maximum primer product length 
Upload custom database file
Select file : 
Select Reference database for Blast search
Only custom database Only NCBI NT Blast database
 
It is recommended to update the local NCBI NT database on monthly basis  
      
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